Transgenic plants for Molecular Farming

The German Television ZDF reported on Sunday May 21 that a German researcher found a gene transfer from genetically engineered rapeseed to bacteria and fungi in the gut of honey bees. Prof. Hans-Hinrich Kaatz from the Institut fur Bienenkunde (Institute for bee research) at the University of Jena experimented during the last three years with honey bees on an experimental field with transgenic rapeseed in Saxony, Germany.

Transgenic plants suitable for food processing

Transgenic animals raise a particularly difficult problem.

The plants oranimals modified in this way are called transgenic.

Before choosing the production system, there are many factors which must be kept in mind while choosing plant species, tissue and target subcellular organelles that will be used as chosen host for production of recombinant proteins. One of the most important factors is the expression level, another one is from the biosafety point of view, the impact of exposure on the environment, food and feed chains. One of the potential risks is the pollen transferability from transgenic to related outcrossing species and other nontransgenic crops which may lead to persistence of genetically engineered material in environment and nontarget organisms[,]. According to overexpression of aglycosylated CTB causes massive tissue necrosis and poor accumulation unless retained in the endoplasmic reticulum (ER)[]. They also reported as, gCTB's potential as an oral immunogen and point to a potential role of N-glycosylation in increasing recombinant protein yields in plants. Some examples of various Bio-pharmaceuticals produced in transgenic plants and in pipeline for commercialization are shown in Table 1.

In favour of transgenic animals:

The first transgenic animals were mice created by Rudolf Jaenisch in 1974. Jaenish successfully managed to insert foreign DNA into the early-stage ; the resulting mice carried the modified gene in all their tissues. Subsequent experiments, injecting leukemia genes to early mouse embryos using a retrovirus vector, proved the genes integrated not only to the mice themselves, but also to their progeny.

For animal welfare concerns regarding the use of transgenic animals,see (optional).

improve plants, trees or animals, ..

Einspanier R, Klotz A, Kraft J et al (2001). European Food Research and Technology Abstract Volume 212 Issue 2 (2001) pp 129-134 The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material

Structural Biochemistry/Nucleic Acid/DNA/Transgenic Plants

Transgenic art acknowledges these changes and atthe same time offers a radical departure from them, placing the questionof actual creation of life at the center of the debate.

PPT – Transgenic Plants PowerPoint presentation | free …

Transgenic animals pose problems for religions that restrict the foods that their believers can eat, since they may produce animals that appear to be one species, but contain some elements of a forbidden species.

Transgenic Animals and Plants Essay - 1373 Words

"GFPBunny" highlights the fact that transgenic animals are regular creaturesthat are as much part of social life as any other life form, and thus aredeserving of as much love and care as any other animal [16].

Transgenic plants, animals, and bacteria - Mega Essays

Microbes in the digested food that had passed through the small intestine were cultured through 6 passages in broth containing glyphosate. Bacteria grew to a density of 108/ml in each sub-culturing. In each sub-culture derived from samples taken from 3 subjects at 180, 240 and 300 min after eating, the transgene was found. This is yet another gross underestimate. The method depends on the bacteria having taken up an intact gene coding for glyphosate tolerance, and would not have detected bacteria that have taken up fragments of that gene, or other parts of the GM DNA containing other genes or gene fragments.

Techniques Used for Generating Transgenic Plants: ..

Placement of the start and stop codons will determine whether the final protein fusion will contain amino acids coded by the recombination sites used in cloning. Ideally the start and stop codons should flank the ORF directly to avoid adding any extensions to the transgenic protein (). In practice though, if using clones from available libraries, you may be forced to consider a different strategy.